Document Type : Research Paper

Abstract

Food preservation has always been a concern for mankind. Different methods have been developed to preserve food for long periods, such as cold storage, plastic covers, and modified atmosphereThis study examined the physicochemical and biological effects of a modified atmosphere on packaged Romaine lettuce at three levels (G1: 40% oxygen, 15% carbon dioxide, 45% nitrogen; G2: 70% oxygen, 15% carbon dioxide, 15% nitrogen; G3: 100% oxygen). The lettuce was packaged in 40 µm (P1) or 50 µm (P2) thick polyethylene at 4°C and stored for 16 days. The least polluted sample was G3 using P2, as assessed by the total number of microorganisms, mold and yeast. This sample was also in the best condition, as measured by pH and color. The highest percentage of weight loss was for G3 and P1; it also showed the greatest decrease in the percentage of soluble solid materials. The results showed that G3 and P1 was the best combination to preserve packaged Romaine lettuce. This combination was effective for two weeks of preservation.

Keywords

Ahn, H. J., Kim, J. H., Kim, J. K., Kim, D. H., Yook, H. S. and Byun M. W. 2005. Combined effects of irradiation and modified atmosphere packaging on minimally processed Chinese cabbage (Brassica rapa L.). Food. Chem. 89(4): 589–597.
Amanatidou, A., Smid, E. J. and Gorris, L. G. M. 1999. Effect of elevated oxygen and carbon dioxide on the surface growth of vegetable- associated micro-organisms. J. Appl. Microbiol. 86(3): 429–438.
Ayhan, Z., Esturk, O. and Tas, E. 2008. Effect of modified atmosphere packaging on the quality and
shelf-life of minimally processed carrots. Turk. J. Agric. For. 32(1): 57-64.
Conesa, A., Artes-Hernandez, F., Geysen, S., Nicolai, B. and Artes, F. 2007a. High oxygen combined with high carbon dioxide improves microbial and sensory quality of fresh-cut peppers. Postharvest. Biol. Technol. 43(2): 230–237.
Conesa, A., Verlinden, B. E., Artes-Hernandez, F., Nicolai, B. and Artes, F. 2007b. Respiration rates of fresh-cut bell peppers under supertamospheric and low oxygen with or without high carbon dioxide. Postharvest. Biol. Technol. 45(1): 81–88.
Das, E., Gurakan, C. and Bayindirl, A. 2004. Effect of controlled atmosphere storage, modified atmosphere packaging and gaseous ozone treatment on the survival of  Salmonella Enteritidis on cherry tomatoes. M. Sc. Thesis. Middle East Technical University. Department of Food Engineering. Ankara. Turkey.
Day, B. P. F. 1996a. Novel MAP for fresh prepared produce. Eur. Food Drink Rev. 1, 73–80.
Day, B. P. F. 1996b. High oxygen modified atmosphere packaging for fresh prepared produce. Postharvest News Info. 7(3): 31–34.
Day, B. P. F.  2000. Novel MAP for freshly prepared fruit and vegetable products. Postharvest News Info. 11, 27–31.
Day, B. P. F. 2001. Fresh Prepared Produce: GMP for High Oxygen MAP and Non-Sulphite Dipping. Guideline. No. 31. Campden and Chorleywood Food Research Association Group. Chipping Campden. Gloshister. UK.
Ding, C. K., Chachin, K., Ueda, Y., Imahori, Y. and Wang C. Y. 2002. Modified atmosphere packaging maintains postharvest quality of loquat fruit. Postharvest. Biol. Technol. 24(3): 341–348.
Dunn, O. J. and Clark, V. A. 1974. Applied Statistics: Analysis of Variance and Regression. John Wiley & Sons Pub. New York.
Fakharian, N., Hassanpour-Asil, M. and Asgari, M. 2008. Effect of modified atmosphere packaging and cold storage on quality and quantity characteristes of two varieties of lettuce (Lactuca sativa L.).  Iranian J. Food Sci. Technol. 5(1): 37-50. (in Farsi)
Gorny, J. 1997. A summary of CA and MA requirements and recommendations for fresh-cut (minimally processed) fruits and vegetables. Proceedings of the 7th International Controlled Atmosphere Research Conference on Fresh-Cut and Vegetables and MAP. July. 13-18. University of California. Department of Pomology. Davis. California. USA. 5, 30–66.
Hamidi-Esfahani, Z. and Tajeddin, B. 1996. Effectives factors on modified atmosphere packaging of fresh vegetable and fruits. Proceeding of the 8th National Iranian Conference of Food Industry. Karaj. Iran. (in Farsi)
Heimdal, H., Kuhn, B. F., Poll, L. and Larsen, L. M. 1995. Biochemical changes and sensory quality shredded and MA-packaged iceberg lettuce. J. Food Sci. 60(6): 1265–1268.
Jacxsens, L., Devlieghere, F., Van der Steen, C. and Debevere, J. 2001. Effect of high oxygen modified atmosphere packaging on microbial growth and sensorial qualities of fresh-cut produce. Int. J. Food microbiol. 71(2): 197–210.
Kader, A. A. and Ben-Yehoshua, S. 2000. Effect of superatmospheric oxygen levels on postharvest physiology and quality of fresh fruits and vegetables. Postharvest Biol. Technol. 20(1): 1–13.
Karim, G. 1991. Microbiological Examination of Food. Tehran University Publication. Tehran. Iran. (in Farsi)
Maskan, M. 2001. Kinetics of colour change of kiwifruits during hot air and microwave drying. J. Food. Eng. 48(2): 169-175.
Nguz, K., Shindano, J., Samapundo, S. and Huyghebaert, A. 2005. Microbiological evaluation of fresh-cut organic vegetables produced in Zambia. Food Control. 16(7): 623-628.
Saxena, A., Bawa, A. S.  Raju, P. S.  2008. Use of modified atmosphere packaging to extend shelf-life of minimally processed jackfruit (Artocarpus heterophyllus L.) bulbs. J. Food Eng. 87(4): 455-466.
Srilaong, V., Kanlayanarat, S. and Tatsumi, Y. 2002. Changes in commercial quality of ‘Rong-Rien’ rambutan in modified atmosphere packaging. Food Sci. Technol. Res. 8(4): 337–341.
Wszelaki, A. L. and Mitcham E. J. 2000. Effects of superatmospheric oxygen on strawberry fruit quality and decay. Postharvest Biol. Technol. 20(2): 125–133.