The Effect of Sourdough on the Microbiological Shelf life and Sensory Properties of Barbari Bread

Document Type : Research Paper

Abstract

This study examined sourdough LAB containing specific starter cultures used to produce barbari-style bread and evaluated their microbiological shelf life and sensory properties. The effects of sourdough fermentation time (8, 16, 24h), fermentation temperature (28, 32, 36°C) and type of starter culture (Lactobacillus sanfransicencis, ATCC 14917; Lactobacillus plantarum, ATCC 43332; a mixture of both LABs) were analyzed in a completely randomized design factorial experiment with four replications. The microbiological shelf life of samples was determined by placing serial dilutions on plate count agar for one to seven days. The sensory properties of the bread were determined 1, 24, 48 and 72h after baking using AOAC standards. The results showed that sourdough had a significant effect (p≤0.05) on improving the microbiological shelf life and sensory properties of barbari bread in comparison with the control sample. Moreover, the sample produced with Lactobacillus plantarum (24h fermentation time and 32ºC fermentation temperature) had the maximum microbiological shelf life and the sample produced with a mixture of both LABs (16h fermentation time and 28ºC fermentation temperature) had the best sensory evaluation 72 h after baking.

Keywords

20.1001.1.26454531.1387.9.3.10.9

References

Anon. 1983. AACC Method 44-15. Approved Methods of the American Association of Cereal Chemists. 8^th Ed. The Association, St. Paul. MN.

Anon. 1990. AOAC Method, In Official methods of analysis. Association of Official Analytical Chemists. 15^th Ed. Arlington. Virginia.

Arendt, E. K., Ryan, L. A. M. and Dal Bello, F. 2007. Impact of sourdough on the texture of bread. Food Micro. 24(2): 165-174.

Dal Bello, F., Clarke, C. I., Ryan, L. A. M., Ulmer, H., Schober, T. J., Ström, K., Sjögren, J., van Sinderen, D., Schnürer, J. and Arendt, E. K. 2007. Improvement of the quality and shelf life of wheat bread by fermentation with the antifungal strain Lactobacillus plantarum FST 1.7. J. Cereal Sci. 45(3): 309-318.

Gianotti, A., Vannini, L., Gobbetti, M., Corsetti, A., Gardini, F. and Guerzoni, M. E. 1997. Modeling of the activity of selected starters during sourdough fermentation. Food Micro. 14(4): 327-337.

Guerzoni, M. E., Vernocchi, P., Ndagijimana, M., Gianotti, A. and Lanciotti, R. 2007. Generation of aroma compounds in sourdough: Effects of stress exposure and lactobacilli–yeasts interactions. Food Micro. 24(2): 139-148.

Gül, H., Özçelik, S., Sa˘gdıç, O. and Certel, M. 2005. Sourdough bread production with lactobacilli and Saccharomyces cerevisiae isolated from sourdoughs. Process Biochemistry. 40, 691-697.

Katina, K. 2005. Sourdough: A tool for the improved flavour, texture and shelf-life of wheat bread. VTT Biotechnology. VTT Technical Research Centre of Finland. No. 569.

Katina, K., Sauri, M., Alakomi, H. L. and Mattila-Sandholm, T. 2002. Potential of lactic acid bacteria to inhibit rope spoilage in wheat sourdough bread. LWT. 35, 38-45.

Katina, K., Heinio, R. L., Autio, K. and Poutanen, K. 2006. Optimization of sourdough process for improved sensory profile and texture of wheat bread. LWT. 39, 1189-1202.

Katina, K., Laitila, A., Juvonen, R., Liukkonen, K. H., Kariluoto, S., Piironen, V., Landberg, R., Man, P. A. and Poutanen, K. 2007. Bran fermentation as a means to enhance technological properties and bioactivity of rye. Food Micro. 24(2): 175-186.

Leroy, F., De Winter, T., Adriany, T., Neysens, P. and De Vuyst, L. 2006. Sugars relevant for sourdough fermentation stimulate growth of and bacteriocin production by lactobacillus amylovorus DCE 471. Int. J. Food Micro. 112, 102-111.

Meignen, B., Onno, B., Ge¨ linas, P., Infantes, M., Guilois, S. and Cahagnier, B. 2001. Optimization of sourdough fermentation with Lactobacillus brevis and baker's yeast. Food Micro. 18, 239-245.

Mentes, O., Ercan, R. and Akcelik, M. 2007. Inhibitor activities of two lactobacillus strains, isolated from sourdough, against rope-forming bacillus strains. Food Control. 18(4): 359-363.

Simsek, O., Hilmi Con, A. and Tulumoglu, S. 2006. Isolating lactic starter cultures with antimicrobial activity for sourdough processes. Food Control. 17, 263-270.

Suhr, K. I. and Nielsen, P. V. 2004. Effect of weak acid preservatives on growth of bakery product spoilage fungi at different water activities and pH values. Int. J. Food Micro. 95, 67-78.

Thiele, C. 2003. Hydrolysis of gluten and the formation of flavor precursors during sourdough fermentation. T. U. M.

Thiele, C., Gänzle, M. G., and Vogel, R. F. 2002. Contribution of sourdough lactobacilli, yeast and cereal enzymes to the generation of amino acids in dough relevant for bread flavor. Cereal Chem. 79, 45-51.

Todorov, S., Onno, B., Sorokine, O., Chobert, J. M., Ivanova, I. and Dousset, X. 1999. Detection and characterization of a novel antibacterial substance produced by Lactobacillus plantarum ST 31 isolated from sourdough. Int. J. Food Micro. 48, 167-177.

Wagner, S. C. 2005. Producing sourdough breads to illustrate the use of industrial microorganisms. Ame. Bio. Teacher. 67(2): 96-101.

Food Engineering Research

The Effect of Sourdough on the Microbiological Shelf life and Sensory Properties of Barbari Bread

References

References

Volume 9, Issue 3 - Serial Number 3
January 0
Pages 153-170

The Effect of Sourdough on the Microbiological Shelf life and Sensory Properties of Barbari Bread

References

References

Volume 9, Issue 3 - Serial Number 3January 0Pages 153-170

Volume 9, Issue 3 - Serial Number 3
January 0
Pages 153-170