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The effect of additives, carriers, and variables of Spray Drying Process on the Stability of Xylanase

Document Type : Research Paper

Authors

1 - Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran - Biotechnology Department, Animal Science Research

2 Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Xylanase is one of the critical enzymes in the food, poultry feed as well as paper industries. This research aims to provide formulation and operating conditions suitable for spray drying of xylanase enzyme while maintaining enzyme activity. For this purpose, the xylanase was produced by Bacillus subtilis D3d strain using Brewers' spent grain (BSG) and yeast extract as carbon and nitrogen source in a 14-liter fermenter. After separating biomass and solid substrate by centrifuge, the supernatant was used as an enzyme source with a xylanase activity of 350 U/ml. Among the additives, manganese ions at a concentration of 10 mM had the most significant effect on increasing enzyme activity. Among the examined carriers, mannitol and magnesium sulfate showed the slightest decrease in enzyme activity. Magnesium sulfate and malt extract were selected as carrier and binder in concentrations of 20 and 5, (w/v) respectively. In the spray drying operation with an inlet temperature of 120°C, and an inlet feed flow rate of 4 ml/min, the enzyme activity was maintained and the powder with the 4.5% of moisture content was obtained.

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References
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Food Engineering Research
Volume 22, Issue 1 - Serial Number 74
Spring and Summer 2023
June 2023
Pages 181-194
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