نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد علوم و صنایع غذایی، دانشکده علوم کشاورزی و صنایع غذایی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران

2 دانشیار گروه علوم و صنایع غذایی، دانشکده علوم کشاورزی و صنایع غذایی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران

3 موسسه تحقیقات فنی و مهندسی کشاورزی، سازمان تحقیقات، آموزش و ترویج کشاورزی. کرج. ایران

چکیده

فسفات‌ها در بسیاری از مواد غذایی فرایند­شده به عنوان پایدارکننده و امولسیفایر به کار می‌روند. از فسفات‌ها در بیش از 65 درصد فراورده‌های گوشتی استفاده می‌شود. با توجه به مخاطرات احتمالی مصرف فسفات‌ها برای سلامتی و نیز با توجه به­تمایل مصرف‌کنندگان به خرید فراورده‌های فاقد فسفات یا با فسفات کاهش‌یافته، در سال‌های اخیر مطالعات متعددی برای جایگزینی فسفات در فراورده‌های گوشتی شده است. به دلیل ویژگی‌های عملکردی منحصر به فرد فسفات‌ها، از لحاظ تکنولوژیکی حذف کامل آنها چالش برانگیز است. هدف از پژوهش حاضر، جایگزینی نسبی فسفات به میزان 50 درصد اولیه، در سوسیس کوکتل با نشاستۀ اصلاح شدۀ سیب‌زمینی است. اثر افزودن سه نوع نشاسته بر ویژگی‌های فیزیکوشیمیایی سوسیس شامل pH، افت پخت، سفتی بافت، ویژگی‌های میکروبی (کلی‌فرم، اشریشیاکلی، استافیلوکوکوس اورئوس، کلستریدیوم پرفرینژنز، سالمونلا و کپک و مخمر) و ویژگی‌های حسی (رنگ، مزه، عطر و بو، بافت و پذیرش کلی) در سیستم هدونیک 5 امتیازی و طی سی روز نگهداری (زمان‌های صفر، 15 و 30 روز بعد از تولید) بررسی شد. این سه نوع نشاسته عبارت­اند از:  استیلیتد دی استارچ آدیپات (Acetylated distarch adipate)، هیدروکسی پروپیل دی استارچ فسفات (Hydroxypropyl distarch phosphate) و استارچ سدیم اکتنیل سوکسینات (Starch sodium octenyl succinate) در دو سطح 1.35 و 2.7 درصد در مقایسه با نمونه‌های سوسیس تولید شده با فسفات تجاری (نمونۀ شاهد- فاقد نشاستۀ اصلاح شده). جایگزین کردن فسفات با نشاستۀ اصلاح شده تفاوت معنی‌داری در pH نمونه‌ها با شاهد ایجاد نکرد. تفاوت معنی‌داری (p<0.05) در میزان افت پخت نمونه‌های حاوی 1.35 درصد نشاسته اصلاح شده، با نمونه شاهد مشاهده نشد. افت پخت در سوسیس‌های حاوی 2.7 درصد نشاسته اصلاح شده که میزان رطوبت بالاتری داشتند، به صورت معنی‌داری بیشتر بود. تمامی نمونه‌ها از نظر میکروبی مطابق استانداردهای ملی ایران بودند. نمونه‌های سوسیس فرموله شده، در زمان صفر تفاوت معنی‌داری (p<0.05)  با شاهد نداشتند. پس از ۳۰ روز نگهداری، بار میکروبی تیمارهای حاوی 1.35 درصد استیلیتد دی استارچ آدیپات و هیدروکسی پروپیل دی استارچ فسفات کمتر از بار میکروبی شاهد بود. افزایش مقدار نشاسته به 2.7 درصد، صرف‌نظر از نوع آنها، باعث افزایش بار میکروبی شد. در این تحقیق مطلوب‌ترین فرمول از نظر کیفی سوسیس‌های حاوی 1.35 درصد نشاسته اصلاح شده هیدروکسی پروپیل دی استارچ فسفات بودند. سوسیس‌های حاوی 2.7 درصد نشاسته اصلاح شده استارچ سدیم اکتنیل سوکسینات در مقایسه با سایر فرمولاسیون‌ها مطلوبیت کمتری داشتند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Relative Phosphate Replacement with Modified Potato Starch in Cocktail Sausage Formulation

نویسندگان [English]

  • Zahra Malekzadeh 1
  • Seyed Ebrahim Hoseini 2
  • Homa Behmadi 3

1 1. MSc of Food Science and Technology, Faculty of Agricultural Science and Food Industrial, Science and

2 Associate Professor of Department of Food Science and Technology, Faculty of Agricultural Science and Food Industrial, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Agricultural Engineering Research Institute, Agriculture,, Research, Education Organization, Karaj. Iran

چکیده [English]

Phosphates are used as stabilizers and emulsifiers in many processed foods. Phosphates are used in more than 65% of meat products. Due to the potential health risks of phosphate consumption and the tendency of consumers to buy phosphate-free or phosphate-reduced products, several studies have been conducted in recent years to replace phosphate in meat products. Due to the unique functional properties of phosphates, their complete removal is technologically challenging. The aim of the present study was to partially replacement of phosphate in cocktail sausages with modified potato starch. Effect of adding three types of starch including: Acetylated distarch adipate, Hydroxypropyl distarch phosphate and Starch sodium octenyl succinate at two levels of 1.35 and 2.7% compared to samples of sausages produced with commercial phosphate (Control sample - no modified starch) on physicochemical properties of sausage including pH, cooking loss, tissue firmness, microbial characteristics (coliform, Escherichia coli, Staphylococcus aureus, Clostridium perfringens, Salmonella and mold and yeast) and sensory characteristics (color, odor, texture and acceptance) were evaluated in a 5-point hedonic system during 30 days of storage (zero, 15 and 30 days after production). Replacement of phosphate with modified starch did not make a significant difference in the pH of the samples with the control. No significant difference (p <0.05) was observed in the cooking loss of samples containing 1.35% of modified starch with the control sample. The cooking loss was significantly higher in sausages containing 2.7% of modified starch with higher moisture content. All samples were microbiologically in accordance with Iranian national standards. Formulated sausage samples were not significantly different (p <0.05) from the control at time zero. After 30 days of storage, the microbial load of treatments containing 1.35% of acetylated diastarch adipate and hydroxypropyl diastarch phosphate were less than the control. Increasing the amount of starch to 2.7%, regardless of their type, increased the microbial load.  In this study, the most desirable formula in terms of quality were sausages containing 1.35% modified hydroxypropyl diastarch phosphate starch and sausages containing 2.7% modified sodium starch actinyl succinate starch were less desirable compared to other formulations.

کلیدواژه‌ها [English]

  • Chemical Additives
  • Reduced Phosphate
  • Water Storage Capacity
  • Meat Products
Abd El-Ghany, N.A. and Mahmoud, Z.M. 2020. Synthesis, characterization and swelling behavior of high-performance antimicrobial amphoteric hydrogels from corn starch. Polymer Bulletin, pp.1-22.
Abd El-Ghany, N.A., Aziz, M.S.A., Abdel-Aziz, M.M. and Mahmoud, Z. 2019. Antimicrobial and swelling behaviors of novel biodegradable corn starch grafted/poly (4-acrylamidobenzoic acid) copolymers. International journal of biological macromolecules. 134, 912-920.
Aktas, N., Genccelep, H. 2006. Effect of starch type and its modifications on physicochemical properties of bologna-type sausage produced with sheep tail fat. Meat Science, 74: 404–408.
Asioli, D., Aschemann-Witzel, J., Caputo, V., Vecchio, R., Annunziata, A., Naes, T. and Varela, P. 2017. Making sense of the "clean label" trends: A review of consumer food choice behavior and discussion of industry implications. Food Research International. 99, 58-71.
Bacchetta, J., Bernardor, J., Garnier, C., Naud, C. and Ranchin, B. 2020. Hyperphosphatemia and chronic kidney disease: a major daily concern both in adults and in children. Calcified tissue international. 108, 116-127.
Cankaya, N. 2018. Modification of starch: structural and antimicrobial properties. International Journal of Chemistry and Chemical Engineering Systems. 3.31-35.
Choi Y.S. Jeong, T.J. Hwang K. E. Song D. H. Ham, Y. K. Kim, Y. B. Jeon, K. H. Kim, H.W. Kim C. J. 2016. Effects of various salts on physicochemical properties and sensory characteristics of cured meat. Korean Journal Food Science Animal Resources. 36 (2): 152–158.
Ciosek, Ż., Kot, K., Kosik-Bogacka, D., Łanocha-Arendarczyk, N. and Rotter, I., 2021. The Effects of calcium, magnesium, phosphorus, fluoride, and lead on bone Tissue. Biomolecules, 11(4): 535-566.
Dürig, T. and Karan, K., 2019. Binders in wet granulation. In Handbook of Pharmaceutical Wet Granulation (pp. 317-349). Academic Press.
Dykes, G. A., Coorey, R., Ravensdale, J. T., & Sarjit, A. 2019. Phosphates. In L. Melton, F. Shahidi, & P. Varelis (Eds.), Encyclopedia of food chemistry (pp. 218-224). Oxford: Academic Press.
Hayes, J.E., StePanyan. V., Allen, P., O’Grady, M.N., & Kerry, J.P. 2011. Evaluation of the effects of selected plant-derived nutraceuticals on the quality and shelf-life stability of raw and cooked pork sausages. LWT-Food Science and Technology, 44, 164-172.
Hughesa, J., Oisethb, S., Purslowc, P. and Warner, R. D. 2014. A structural approach to understanding the interactions between colour, water-holding capacity and tenderness. Meat Science. 98 (3): 520-532.
Institute of Standard and Industrial Researchs of Iran. 2007b. Microbiology of food and animal feed –Comprehensive method for counting coliform bacteria - colonycounting method. Iran National Standard. No. 9263. (in Persian)
Institute of Standard and Industrial Researchs of Iran. 2008b. Microbiology of food and animal feed- search Comprehensive method for counting Moulds and Yeasts colony count technique at 25°C. Iran National Standard.NO.10899-1. (in Persian)
Institute of Standard and Industrial Researchs of Iran.2002. Microbiology of food and animal feed feeding stuffs- Horizontal method for the detection of salmonella. Iran National Standard. No. 1810. (in Persian)
Institute of Standard and Industrial Researchs of Iran.2005a.Microbiology of food and animal feed - search and counting of Escherichia coli using the most probable number method, Iran National Standard. No. 2946. (in Persian)
Institute of Standards and Industrial Research of Iran. 2005b. Microbiology of food and animal feeding stuffs – Enumeration of coagulase – Positive staphylococci (staphylococcus aureus and other species) – Test method. Part 1: 2nd revision. Iran National Standard .No.6806-1. (in Persian)
Institute of Standards and Industrial Research of Iran. 2007a. Microbiology of food and animal feeding Stuffs – Horizontal method for the enumeration of microorganisms – Colony count technique at 30°C. Iran National  Standard No. 5272. (in Persian)
Institute of Standards and Industrial Research of Iran. 2008a. Meat and meat products – Measurement of pH – Reference test method. 1st Revision, ISIRI No. 1028. (in Persian)
Institute of Standards and Industrial Research of Iran.2006. Microbiology of food and animal feeding stuffs - Horizontal method for enumeration of clostridium perfringens colony count technique. Iran National Standard. No. 2197. (in Persian)
Jo, K., Lee, S., Jo, C., Jeon, H.J., Choe, J.H., Choi, Y.S. and Jung, S., 2020. Utility of winter mushroom treated by atmospheric non-thermal plasma as an alternative for synthetic nitrite and phosphate in ground ham. Meat science, 166, p.108151.
Kalantar-Zadeh, K., Gutekunst, L., Mehrotra, R., Kovesdy, C. P., Bross, R., Shinaberger, C. S., Kopple, J. D. 2010. Understanding sources of dietary phosphorus in the treatment of patients with chronic kidney disease. Clinical Journal of the American Society of Nephrology. 5(3), 519- 673 530
Kumar, M. and Sharma, B.D. 2003. Quality characteristics of low-fat ground pork patties containing milk co-precipitate. Asian-australasian journal of animal sciences, 16(4): 588-595.
Leon, J. B., Sullivan, C. M., & Sehgal, A. R. 2013. The prevalence of phosphorus-containing food additives in top-selling foods in grocery stores. Journal of Renal Nutrition, 23(4): 265-U230.
Lu, F., Kang, Z.L., Wei, L.P. and Li, Y.P. 2021. Effect of sodium bicarbonate on gel properties and protein conformation of phosphorus-free chicken meat batters. Arabian Journal of Chemistry, 14(2): p.102969.
Mbougueng, P. D., Tenin, D., Tchiégang, C. and Scher J. 2015. Effect of starch type on the physicochemical and textural properties of beef patties formulated with local spices. American Journal of Food Science and Technology. 3: 2, 33-39.
Motzer, E.A., Carpenter, J.A., Reynolds, A.E. and Lyon, C.E., 1998. Quality of restructured hams manufactured with PSE pork as affected by water binders. Journal of Food Science. 63(6): 1007-1011.
Nielsen, G. S., Peterson, B. R. and Moller, A. J. 1995. Impact of salt, phosphate and temperature on the effect of a transglutaminase (F XIIIa) on the texture of restructured meat. Meat Science, 41: 293–299.
Peleg, M., Corradini, M. G., and Normand, M. D. 2015. On modeling the effect of water activity on microbial growth and mortality kinetics. Pharmaceutical and Food Systems, 263-278.
Pereira, J., Hu, H., Xing, L., Zhang, W. and Zhou, G. 2020. Influence of rice flour, glutinous rice flour, and tapioca starch on the functional properties and quality of an emulsion-type cooked sausage. Foods, 9(1), p.9.
Petridis, D., Ritzoulis, C., Tzivanos, I., Vlazakis E., Derlikis E. and Vareltzis P. 2013. Effect of fat volume fraction, sodium caseinate, and starch on the optimization of the sensory properties of frankfurter sausages. Food science and Nutrition. 1 (1): 32-44.
Pietrasik, Z. 1999. Effect of content of protein, fat and modified starch on binding textural characteristics, and colour of comminuted scalded sausages. Meat Science, 51: 17-25.
Pinton, M.B., dos Santos, B.A., Lorenzo, J.M., Cichoski, A.J., Boeira, C.P. and Campagnol, P.C.B., 2021. Green technologies as a strategy to reduce NaCl and phosphate in meat products: An overview. Current Opinion in Food Science. 40, 1-5.
Prabhu, G. Husak, R. 2014. Use of sodium carbonate and native potato starch blends as a phosphate replacer in natural enhanced pork loins. Meat Science. 96(1): 454-455.
Razzaque, M.S. 2020. Can excessive dietary phosphate intake influence oral diseases? Advances in Human Biology. 10(2): 35-37.
Regulation (EC) No. 1333/2008 of the European Parliament and of the council of 16 December 2008 on food additives. In: Official Journal of the European Union https://ec.europa.eu/food/safety/food_improvement_agents/additives/database_en.
Resconi V. C., Keenan D. F., García E., Allen P., Kerry J. P. and Hamill R. M. 2016. The effects of potato and rice starch as substitutes for phosphate in and degree of comminution on the technological, instrumental and sensory characteristics of restructured ham. Meat Science. 121, 127-134.
Riis, J., Loewenstein, G., Baron, J., Jepson, C., Fagerlin, A. and Ubel, P. A. 2005. Ignorance of hedonic adaptation to hemodialysis: a study using ecological momentary assessment. Journal of Experimental Psychology: General. 134(1): 3-9.
Ruusunen, M., Vainionpää, J., Lyly, M., Lähteenmäki, L., Niemistöc, M., Ahvenainen, R., & Puolanne, E. 2005. Reducing the sodium content in meat products: the effect of the formulation in low-sodium ground meat patties. Meat Science, 69, 53–60.
Schutte, S., Marais, J., Muller, M. and Hoffman, L.C. 2021. Replacement of Sodium Tripolyphosphate with Iota Carrageenan in the Formulation of Restructured Ostrich Ham. Foods. 10(3): 535-546.
Thangavelu, K.P., Kerry, J.P., Tiwari, B.K. and McDonnell, C.K. 2019. Novel processing technologies and ingredient strategies for the reduction of phosphate additives in processed meat. Trends in Food Science & Technology. 94, 43-53.
Wang, Y., Sun, Y., Zhang, X., Zhang, Z., Song, J., Gui, M., and Li, P. 2015. Bacteriocin-producing probiotics enhance the safety and functionality of sturgeon sausage. Food Control. 50, 729-735.
Wyers, R. 2019. Blog: EFSA issues new phosphate limits advice, warns that safe levels may be being exceeded. Food Ingredients 1st .Available at: https://www.foodingredientsfirst.com/news/efsa-issues-new-eu-phosphates-limits-advice warns-that-safe-levels-may-be-being-exceeded.html