نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم و مهندسی صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران

2 گروه صنایع غذایی دانشکده علوم کشاورزی و صنایع غذایی واحد علوم و تحقیقات

3 گروه شیلات، دانشکده علوم و فنون دریایی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران

چکیده

گیاهان آبزی یکی از منابع مهم ترکیبات زیست فعال با خاصیت آنتی اکسیدانی محسوب می‌شوند. در تحقیق حاضر به بهینه سازی استخراج ترکیبات آنتی اکسیدانی شامل کلروفیل a و b، کاروتنوئید و ترکیبات فنلی از جلبک Laurencia papillosa با روش مایکروویو پرداخته شده است که براساس متغیرهای مستقل شامل زمان استخراج، توان مایکروویو، نوع حلال و نسبت حلال به نمونه است. برای طراحی آزمایش در این مطالعه از نرم افزار Design Expert  با 25 اجرا استفاده و مقدار ترکیبات آنتی اکسیدانی جلبک اندازه گیری شد. نتایج حاصل از سنجش کلروفیل a و b حاکی از آن است که هیچ یک از چهار متغیر مستقل گفته‌شده اثر معنی‌داری(P>0.05) بر استخراج کلروفیل نداشته است. در مورد کاروتنوئید، فاکتورهای زمان، اثر متقابل زمان×حلال و اثر متقابل زمان×توان دارای اثر معنی‌دار (P>0.05)بوده‌اند و طبق نتایج به‌دست آمده شرایط بهینه استخراج کاروتنوئید شامل حلال متانول با نسبت 10.5 به 1، توان 156 وات و زمان 24.5 دقیقه بوده است. در زمینه ترکیبات فنلی، فاکتورهای حلال، زمان، اثر متقابل حلال×زمان و اثر متقابل حلال×توان دارای اثر معنی‌دار  (P>0.05) بوده‌اند و حلال متانول با نسبت 12.5 به 1، توان 180 وات و زمان 10 دقیقه شرایط بهینه استخراج فنل را تشکیل داده‌اند. برابر داده‌های به‌دست‌آمده از این تحقیق، جلبک Laurencia papillosa خاصیت آنتی اکسیدانی قابل توجهی دارد و با کنترل پارامترهای اثرگذار در فرآیند استخراج با مایکروویو می‌توان به حداکثر میزان ترکیبات آنتی اکسیدانی دست یافت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Extraction Optimization of Antioxidant and Phenolic Compounds of Laurencia papillosa by Microwave-Assisted Process

نویسندگان [English]

  • Yasamin Fayaz 1
  • Nargess Moorki 3

1 Department of Food Science and Technology, Islamic Azad University, Science and Research Branch, Tehran, Iran

3 Department of Fisheries Science, college of Marine Science and Technology, Tehran North Branch, Islamic Azad University, Tehran, Iran

چکیده [English]

Aquatic plants are one of the most important sources of bioactive compounds with antioxidant activity. The aim of this paper is to optimize the microwave extraction of antioxidant compounds including chlorophyll a and b, carotenoids, and phenolic compounds from Laurencia papillosa based on extraction time, microwave power, solvent type, and solvent to sample ratio. Experimental design was performed using Design Expert with 25 runs, and the amounts of the mentioned antioxidant compounds were measured. The results for chlorophyll a and b showed that none of the extraction factors had a significant effect (P > 0.05) on these two types of chlorophylls. In relation to carotenoid extraction, the time, time and solvent interaction, and time and power interaction had significant effects (P 0.05), and according to the results, the optimum conditions for carotenoid extraction include methanol as the solvent with a ratio of 10.5 to 1, power of 156 watts, and time of 24.5 minutes. Regarding phenolic compounds, solvent, time, solvent and time interaction, and solvent and power interaction had significant effects (P 0.05), and the optimum conditions of phenol extraction include methanol as the solvent with a ratio of 12.5 to 1, power of 180 watts, and time of 10 minutes. The results of this paper indicate that Laurencia papillosa has significant antioxidant properties, and by controlling the effective parameters in the microwave extraction process, the maximum amount of antioxidant compounds can be obtained.
 

کلیدواژه‌ها [English]

  • Aquatic plant
  • Bioactive
  • Chlorophyll
  • Carotenoid
  • Solvent
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