تحقیقات مهندسی صنایع غذایی

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بررسی ویژگی‌های دینامیکی و فیزیکوشیمیایی نانو ذرات حاوی عصاره پروپولیس

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 موسسه پژوهشی علوم و صنایع غذایی

چکیده

پروپولیس سرشار از ترکیبات بیواکتیو ازجمله ترکیبات آنتی‌اکسیدان و ضد میکروبی است، ازاین‌رو محافظت از این ترکیبات حساس با روش‌هایی با کارایی بالا مانند ریزپوشانی ضروری است. هدف از این پژوهش، استخراج عصاره پروپولیس، تهیه نانو ذرات حاوی عصاره و بررسی ویژگی‌های فیزیکوشیمیایی آن است. در این پژوهش، عصارۀ پروپولیس با حلال‌های اتانول- آب (70:30 حجمی/حجمی) استخراج و نانو ذرات پروتئینی حاوی آن تهیه شد. ویژگی‌های دینامیکی نور، کارایی ریزپوشانی، تشخیص ساختار کریستالی و آمورف و واکنش‌های موجود بین هسته و مواد دیواره‌ای در نانو ذرات اندازه‌گیری شد. برای تجزیه و تحلیل نتایج از روش آنالیز واریانس، با استفاده از نرم‌افزار SPSS، و برای تحلیل نمودارهای FT-IR از نرم‌افزار IRpal 2. استفاده شد. نتایج به‌دست‌آمده از آزمون دینامیکی و کارایی ریزپوشانی نشان داد که با افزایش مقدار هسته و مواد دیواره‌ای کارایی ریزپوشانی، اندازۀ ذرات و درصد پراکندگی افزایش می‌یابد. در فرمولاسیون با مقادیر یکسان پروتئین، با افزایش غلظت عصارۀ پروپولیس، مقدار بار منفی پتانسیل زتا نیز افزایش یافت. نتایج حاصل از رنگ‌سنجی نشان داد که تیمارهای P6 و P8 به ترتیب بالاترین شاخص * L و * a را داشتند. نمودارهای حاصل از آزمون FT-IR نشان داد که تغییرات بسیار جزئی به صورت جابه‌جایی پیک یا تغییر متقارن در نانو ذرات حاوی عصارۀ پروپولیس نسبت به نانو ذرات شاهد صورت گرفته است. این پژوهش نشان داد در تهیۀ نانو ذرات، هر چه مقدار مادۀ هسته‌ای بیشتر باشد اندازۀ نانو ذرات حاصل بزرگ‌تر خواهد شد و شدت کریستالی و کارایی ریزپوشانی بیشتری نیز حاصل می‌شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of dynamic and physicochemical properties of loaded- propolis extract nanoparticles

نویسندگان [English]

  • Mansooreh Soleimanifard 1
  • Javad Feizy 2

1 Gorgan university of agricultural sciences and natural resources

2 Assistant Professor, Department of Food Safety and Quality Control, Research Institute of Food Science and Technology, Mashhad, Iran.

چکیده [English]

Propolis is rich in bioactive compounds, including antioxidant and antimicrobial compounds, therefore it is necessary to protect these sensitive compounds by high-performance methods such as encapsulation. The purpose of this research was to produce nanoparticles containing the propolis extract and to investigate its physicochemical characteristics. In this research, propolis was extracted with ethanol-water (70:30) solvents and protein nanoparticles containing it were prepared. Then, the dynamic light scattering characteristics, the encapsulation efficiency, the detection of crystalline and amorphous structure, and the interactions between the core and wall materials in nanoparticles were measured. To analyse the results, analysis of variance method was used, using SPSS software. The results of the dynamic test and encapsulation efficiency showed that with the increase in the amount of core and wall materials, the encapsulation efficiency, particle size and PDI increase. In the formulation with the same amounts of protein, with the increase in the concentration of propolis extract, the amount of negative charge of zeta potential also increased. The results of the XRD device showed that the treatments, 6 and 8, had the highest values of L and a, respectively. The graphs obtained from the FT-IR test showed that there were very minor changes in the form of peak displacement or symmetrical changes in the nanoparticles containing propolis extract compared to the control nanoparticles. This research showed that in the preparation of nanoparticles, the larger the amount of nuclear material, the larger the size of the resulting nanoparticles, the crystal intensity and the encapsulation efficiency.

کلیدواژه‌ها [English]

  • Encapsulation
  • Propolis extract
  • Nanoparticles
  • Physicochemical properties
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تحقیقات مهندسی صنایع غذایی
دوره 22، شماره 2 - شماره پیاپی 75
پاییز و زمستان 1402
دی 1402
صفحه 55-68
فایل ها
هم رسانی
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