نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم و صنایع غذایی، دانشکده علوم زیستی، واحد تهران شمال،، دانشگاه آزاد اسلامی ،تهران، ایران

2 دانشگاه تهران

3 گروه بهداشت و کنترل مواد غذایی، دانشکده دامپزشکی، دانشگاه تهران ،تهران، ایران

10.22092/fooder.2026.372953.1459

چکیده

این مطالعه با هدف بررسی اثر ضد میکروبی فیلم نانوکیتوزانکربوکسی ‌متیل سلولز حاوی نانو ذرات نقره و اسانس خوشاریزه (Echinophora sibthorpiana) علیه برخی باکتری‌های مهم غذازاد به­اجرا در آمد. اسانس خوشاریزه از اندام ‌های هوایی گیاه با روش تقطیر با آب و دستگاه کلونجر استخراج شد. نانو ذرات نقره به روش زیستی تهیه شد و اسانس ها با غلظت مشخص اضافه گردید. فیلم‌های نانوکیتوزانکربوکسی ‌متیل سلولز در چهار تیمار شامل: پایه، حاوی نانو نقره، حاوی نانو نقره و ۳/۰ درصد اسانس، و حاوی نانو نقره و ۶/۰ درصد اسانس ساخته شدند. اثر ضد میکروبی این فیلم‌ها علیه سالمونلا تیفی موریوم (Salmonella typhimurium )، اشریشیا کلی (Escherichia coli)، لیستریا مونوسیتوژنز (Listeria monocytogenes) و استافیلوکوکوس اورئوس (Staphylococcus aureus ) ارزیابی شد. داده‌ها با نرم افزار SPSS 26 و آزمون‌ های ANOVA و توکی تحلیل شدند. نتایج نشان داد با افزودن نانو نقره و پس از آن اسانس خوشاریزه به فیلم پایه، قطر هاله عدم رشد افزایش می‌یابد. بیشترین اثر ضد میکروبی در فیلم حاوی ۶/۰ درصد اسانس خوشاریزه مشاهده شد و باکتری‌های گرم ‌مثبت نسبت به گرم ‌منفی‌ها حساسیت بیشتری نشان دادند. نتایج تحقیق نشان داد که نانو ذرات نقره و اسانس خوشاریزه موجب افزایش فعالیت ضد میکروبی فیلم‌های نانو کیتوزان-کربوکسی ‌متیل سلولز در شرایط آزمایشگاهی می­شوند. برای معرفی این فیلم‌ها به‌ عنوان گزینۀ کاربردی در بسته‌بندی فعال مواد غذایی، لازم است آزمون‌ های تکمیلی روی مادۀ غذایی صورت پذیرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Antimicrobial Activity of Nanochitosan – Carboxymethyl Cellulose Films Incorporating Silver Nanoparticles and Echinophora sibthorpiana Essential Oil

نویسندگان [English]

  • Allahyar Nabipour 1
  • Zhaleh Khoshkhoo 1
  • Afshin Akhoondzadeh Basti 3
  • Marjaneh Sedaghati 1

1 Department of Food Science and Technology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran

2

3 Department of Food Hygiene and Quality Control,Faculty of Veterinary medicine,Tehran University,Tehran,Iran

چکیده [English]

This study aimed to investigate the antimicrobial effects of nano-chitosan/carboxymethyl cellulose (CMC) films incorporated with silver nanoparticles (SNPs) and Echinophora sibthorpiana essential oil against several foodborne pathogens. The essential oil was extracted from the aerial parts of the plant by hydrodistillation using a Clevenger-type apparatus. Subsequently, silver nanoparticles were synthesized via a green method, followed by the incorporation of the essential oil at specific concentrations. Nano-chitosan/CMC films were prepared in four treatments: control (base film), film containing SNPs, film containing SNPs + 0.3% essential oil, and film containing SNPs + 0.6% essential oil. The antimicrobial activity of these films was evaluated against Salmonella typhimuriumEscherichia coliListeria monocytogenes, and Staphylococcus aureus. Data were analyzed using SPSS software (version 26) through one-way ANOVA and Tukey’s test. The results demonstrated that the incorporation of silver nanoparticles and, subsequently, the essential oil into the base film significantly increased the inhibition zone diameters. The highest antimicrobial activity was observed in the film containing 0.6% essential oil, and Gram-positive bacteria were more sensitive than Gram-negative strains. The results demonstrated that the incorporation of silver nanoparticles and Echinophora sibthorpiana essential oil enhances the antimicrobial activity of chitosan-carboxymethyl cellulose nanofilm. The results indicated that the incorporation of silver nanoparticles and Echinophora sibthorpiana essential oil enhanced the antimicrobial activity of chitosan-carboxymethyl cellulose nanofilms under in vitro conditions. Furthermore, to introduce these films as practical options for active food packaging, additional in situ testing within real food matrices is required.

کلیدواژه‌ها [English]

  • Antimicrobial activity
  • Carboxymethyl cellulose
  • Echinophora sibthorpiana
  • Nanochitosan
  • Silver nanoparticles
Afifi, M. M., & Bagherikia, H. (2025). Application of chitosan edible coating containing Ziziphora clinopodioidesessential oil to extend the shelf life of chicken fillets at inappropriate refrigerated temperature critical conditions. Nurse Physician Within War, 13(46), 28–35.
Asghari, G., Abedi, D., Jalali, M., & Farsi, S. (2007). Antimicrobial activities and phytochemical composition of Echinophora platyloba DC. essential oils from Isfahan. Journal of Essential Oil Bearing Plants, 10(1), 76–82.
Avjigan, M., Saadat, M., NilfrooshZadeh, M. A., & Hafizi, M. (2006). Anti-fungal effect of Echinophora platylobaextract on some common dermatophytes. Journal of Herbal Drugs, 5(18), 10–16.
Baldwin, E. A., Hagenmaier, R., & Bai, J. (Eds.). (2011). Edible coatings and films to improve food quality. CRC Press.
Barzegar Mohammadi, K. , Khanjari, A. , Akhondzadeh Basti, A. , Madani, S. A. and Fayazfar, S. (2025). The Hygienic assessment of Tehran universities food courtsin 2023. Food Engineering Research24(2), 15-22. 
Coma, V. (2008). Bioactive packaging technologies for extended shelf life of meat-based products. Meat Science, 78(1–2), 90–103. https://doi.org/10.1016/j.meatsci.2007.07.035
Deng, W., Liu, K., Cao, S., Sun, J., Zhong, B., & Chun, J. (2020). Chemical composition, antimicrobial, antioxidant, and antiproliferative properties of grapefruit essential oil prepared by molecular distillation. Molecules, 25(1), 217.
Ediyilyam, S., George, B., Shankar, S. S., Dennis, T. T., Wacławek, S., Černík, M., & Padil, V. V. T. (2021).Chitosan/gelatin/silver nanoparticles composites films for biodegradable food packaging applications. Polymers, 13(11), 1680. https://doi.org/10.3390/polym13111680
El-Nashar, H. A., Eldehna, W. M., Al-Rashood, S. T., Alharbi, A., Eskandrani, R. O., & Aly, S. H. (2021). GC/MS analysis of essential oil and enzyme inhibitory activities of Syzygium cumini (Pamposia) grown in Egypt: Chemical characterization and molecular docking studies. Molecules, 26(22), 6984.
Fazlara, A., Pourmahdi, M., Zarei, M., & Karimi, T. (2017). Effect of edible chitosan-rosemary coating on quality and shelf life of refrigerated chicken fillets. Iranian Veterinary Journal, 13(1), 78–90.
Ferhat, M. A., Meklati, B. Y., Smadja, J., & Chemat, F. (2006). An improved microwave Clevenger apparatus for distillation of essential oils from orange peel. Journal of Chromatography A, 1112(1–2), 121–126.
Firoozi, S., Jamzad, M., & Yari, M. (2016). Biologically synthesized silver nanoparticles by aqueous extract of Satureja intermedia CA Mey and the evaluation of total phenolic and flavonoid contents and antioxidant activity. Journal of Nanostructure in Chemistry, 6, 357–364.
Golbashy, M., & Alizadeh Behbahani, B. (2026). Green synthesis of silver nanoparticles using aqueous extract of dill leaves and evaluation of its antibacterial activity. Journal of Food Science and Technology (Iran), 23(172), 62–71.
Hashemi, M., Ehsani, A., Hosseini Jazani, N., Aliakbarlu, J., & Mahmoudi, R. (2013). Chemical composition and in vitro antibacterial activity of essential oil and methanol extract of Echinophora platyloba D.C. against some food-borne pathogenic bacteria. Veterinary Research Forum, 4(2), 123–127. 
Hematizad, I., Khanjari, A., Basti, A. A., Karabagias, I. K., Noori, N., Ghadami, F., et al. (2021). In vitro antibacterial activity of gelatin-nanochitosan films incorporated with Zataria multiflora Boiss essential oil and its influence on microbial, chemical, and sensorial properties of chicken breast meat during refrigerated storage. Food Packaging and Shelf Life, 30, 100751.
Hernández, H., Claramount, D., Kučerová, I., & Banout, J. (2017). The effects of modified blanching and oregano essential oil on drying kinetics and sensory attributes of dried meat. Journal of Food Processing and Preservation, 41(5), e13155.
Jenabi, M., Naghadehi, M. N., & Mashak, Z. (2024). Evaluation of antioxidant and antimicrobial properties of gelatin/chitosan film activated with nano-emulsion of Eryngium platyloba extract against in vitro foodborne pathogens. Journal of Veterinary Microbiology, 20(1), 117–132.
Jiang, B., Wang, F., Liu, L., Tian, S., Li, W., Yang, X., et al. (2017). Antibacterial activity and action mechanism of the Echinops ritro L. essential oil against foodborne pathogenic bacteria. Journal of Essential Oil Bearing Plants, 20(5), 1172–1183.
Kim, K. W., Min, B. J., Kim, Y. T., Kimmel, R. M., Cooksey, K., & Park, S. I. (2011). Antimicrobial activity against foodborne pathogens of chitosan biopolymer films of different molecular weights. LWT - Food Science and Technology, 44(2), 565–569.
Kumar, S., Basumatary, I. B., Sudhani, H. P. K., Bajpai, V. K., Chen, L., Shukla, S., & Mukherjee, A. (2021).Plant extract mediated silver nanoparticles and their applications as antimicrobials and in sustainable food packaging: A state-of-the-art review. Trends in Food Science & Technology, 112, 651–666. 
Lok, C. N., Ho, C. M., Chen, R., He, Q. Y., Yu, W. Y., & Sun, H. (2006). Proteomic analysis of the mode of antibacterial action of silver nanoparticles. Journal of Proteome Research, 5, 916–924.
Maciel, M. V. O. B., da Rosa Almeida, A., Machado, M. H., de Melo, A. P. Z., da Rosa, C. G., de Freitas, D. Z., et al. (2019). Syzygium aromaticum L. (clove) essential oil as a reducing agent for the green synthesis of silver nanoparticles. Open Journal of Applied Sciences, 9(2), 45–54.
Mekonnen, T., Mussone, P., & Bressler, D. (2023). Advancements in carboxymethyl cellulose-based films for food packaging applications. Carbohydrate Polymers, 297, 120668.
Morais, L. D. O., Macedo, E. V., Granjeiro, J. M., & Delgado, I. F. (2020). Critical evaluation of migration studies of silver nanoparticles present in food packaging: A systematic review. Critical Reviews in Food Science and Nutrition, 60(18), 3083–3102.
Newell, D. G., Koopmans, M., Verhoef, L., Duizer, E., Aidara-Kane, A., & Sprong, H. (2010). Food-borne diseases—The challenges of 20 years ago still persist while new ones continue to emerge. International Journal of Food Microbiology, 139, S3–S15.
Pilevar, Z., & Hosseini, H. (2013). Chemical composition, antimicrobial and antioxidant activity of Echinophora platyloba DC. Journal of Pharmacy & Nutrition Sciences, 3(4), 270–283.
Pilevar, Z., Martirosyan, D., Ranaei, V., Taghizadeh, M., Balasjin, N. M., Ferdousi, R., & Hosseini, H. (2024). Biological activities, chemical and bioactive compounds of Echinophora platyloba DC: A systematic review. Bioactive Compounds in Health and Disease, 7(2), 95–109. https://doi.org/10.31989/bchd.v7i2.1283
Raghav, P. K., Agarwal, N., & Saini, M. (2016). Edible coating of fruits and vegetables: A review. Education, 1(2), 188–204.
Rajan, R., Chandran, K., Harper, S. L., Yun, S. I., & Kalaichelvan, P. T. (2015). Plant extract synthesized silver nanoparticles: An ongoing source of novel biocompatible materials. Industrial Crops and Products, 70, 356–373. https://doi.org/10.1016/j.indcrop.2015.03.015
Ramezani, E., Yoosefi, M., Eghbali, S., Shafaei, E., & Tavakoli Kareshk, A. (2024). Investigating the antibacterial effects of synthesized silver nanoparticles Murry plant using the extract of Lycium ruthenicumPars Journal of Medical Sciences, 22(2), 24–35.
Saei-Dehkordi, S. S., Fallah, A. A., Saei-Dehkordi, S., & Kousha, S. (2012). Chemical composition and antioxidative activity of Echinophora platyloba DC. essential oil, and its interaction with natural antimicrobials against food-borne pathogens and spoilage organisms. Journal of Food Science, 77(11), M631–M637. https://doi.org/10.1111/j.1750-3841.2012.02956.x
Sánchez-González, L., Vargas, M., González-Martínez, C., Chiralt, A., & Cháfer, M. (2011). Use of essential oils in bioactive edible coatings. Food Engineering Reviews, 3(1), 1–16. https://doi.org/10.1007/s12393-010-9031-3
Shahidi, F., & John, J. A. (2010). Oxidation and protection of nuts and nut oils. In F. Shahidi (Ed.), Oxidation in foods and beverages and antioxidant applications (Vol. 2, pp. 274–305). Woodhead Publishing. https://doi.org/10.1533/9780857090331.2.274
Shahnia, M., & Khaksar, R. (2013). Antimicrobial effects and determination of minimum inhibitory concentration (MIC) methods of essential oils against pathogenic bacteria. Iranian Journal of Nutrition Sciences & Food Technology, 7(5), 949–955.
Şahin, O., Kanbolat, Ş., Çolak, N. U., Badem, M., Subaş, T., Şener, S. Ö., Korkmaz, N., Kalaycıoğlu, A. T., Öztürk, E., Sayım, A. Ü., Aliyazıcıoğlu, R., Özgen, U., & Kandemir, A. (2025). Investigation of antioxidant and enzyme inhibitory activities of Echinophora chrysantha: Microwave-assisted hydrodistillation and SPME analysis of its essential oils. Chemistry & Biodiversity, 22(2), e202401366. https://doi.org/10.1002/cbdv.202401366
Tauxe, R. V. (2002). Emerging foodborne pathogens. International Journal of Food Microbiology, 78(1), 31–41.
World Health Organization. (2024). Food safety. World Health Organization.
Zhang, Y., Liu, P., & Feng, X. (2024). Characterization and enhancement of carboxymethyl cellulose edible films: A comprehensive review. International Journal of Biological Macromolecules, 234, 127582